Maxima™ H Minus First Strand cDNA Synthesis Kits

Supplier: Fermentas (Thermo Scientific)
Fermentas
K1652EA 572 EUR
K1652 K1681 K1651 K1682
Maxima™ H Minus First Strand cDNA Synthesis Kits
Nucleic Acid Reagents RT-PCR and cDNA Reagents
  • Integrated genomic DNA removal step
  • Increased reaction temperatures: The first strand of cDNA can be synthesised within the 42 to 65 °C temperature range
  • High yields of full-length first strand cDNA with RNA templates up to 20 kb
  • Flexible priming: Oligo(dT)18, random hexamer, or gene-specific primers

Maxima™ H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Delivery information: Maxima™ H Minus First Strand cDNA Synthesis Kit contains Maxima™ H Minus Enzyme Mix, Oligo(dT)18 and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water. Maxima™ H Minus First Strand cDNA Synthesis Kit with dsDNase, in addition to the above listed components, contains dsDNase and 10X dsDNase Buffer.
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