Ethidium bromide 0,625 mg/l in aqueous solution, Biotechnology Grade
Supplier: VWR Chemicals
Ethidium bromide solution is a fluorescent stain for visualising nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0,625 mg/ml. A final working concentration of 0,5 g/ml is easily obtained by adding one drop of solution to 50 ml of molten agarose, running buffer or staining buffer. Ethidium bromide solution in dropper bottles may be stored at room temperature for up to one year.
- Used to detect as little as 10 ng of nucleic acid
- Dropper bottles are shatter-resistant and light-proof
- No additional hazardous shipping costs are applied to ethidium bromide dropper bottles
At a concentration of 0,5 μg/ml, ethidium bromide is useful in agarose or acrylamide gels for band detection.
At higher concentrations (0,5 to 1,0 μg/ml), it may be used to facilitate purification of DNA in a caesium chloride gradient.
One drop diluted into 50 ml of molten agarose or buffer yields a final concentration of 0,5 μg/ml.
Packaging: In 0,625 mg/ml dropper bottles.
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Formula:
C₂₁H₂₀BrN₃ MW: 394,31 g/mol |
MDL Number:
MFCD00011724 EINECS: 214-984-6 |
Specification Test Results
| Abs.@ Lambda Max | 0.68 – 0.74 |
| DNase | none detected |
| Lambda Max (8/100 ml Water) | 478 – 482 nm |
| RNase | none detected |
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