For the generation of microarray probes by either post-labelling (HyPer™5 NHS reactive dye) or direct enzymatic incorporation with HyPer™5 dCTP in gene expression or aCGH experiments.
- Photo-stable and resistant to signal loss upon exposure to light, ozone, and repeated scanning
- High labelling efficiency, consistency, and reproducibility with both tRNA and mRNA inputs
- Comparable signal level to Cy™3 in expression arrays from both direct and post-labeling experiments
- Suitable for using existing labeling methods and filter settings on imaging instruments
- More resistant to environmental conditions than other commercially available microarray dyes
Amersham™ HyPer™5 dye is a fluorescent dye for microarray applications that combines exceptional photo stability and ozone stability with highly reproducible performance. Amersham™ HyPer™5 dyes are available in two formats: first, as a HyPer™5 NHS reactive dye for coupling to aminoallyl modified-cDNA or amplified RNA in post-labelling experiments; second, as a HyPer™5-dCTP for direct enzymatic incorporation and probe labelling reactions.
Amersham™ HyPer™5 dye can be readily incorporated to generate labelled probes from both total and messenger RNA with efficiencies similar to that of Cy™5 dye. Upon microarray hybridisation, Amersham™ HyPer™5 dye demonstrates vastly increased photo stability and ozone stability relative to Cy™5. Array signals from Amersham™ HyPer™5 dyes are resistant to elevated ozone levels and repeated scanning of slides.
Microarray hybridisation with Amersham™ HyPer™5 dye allows gene expression levels to be compared relative to Cy™3 on the same arrays. Amersham™ HyPer™5 dye allows microarray experiments to be performed under any environmental condition using existing labelling methods and filter settings on imaging instruments.
