The Direct cDNA Sequencing Kit enables the identification and quantification of full-length transcripts, without PCR bias, using nanopore sequencing.
- Identify and quantify full-length transcripts
- Explore differential gene expression
- Characterise and quantify isoforms, splice variants, and fusion transcripts
- Avoid bias and artefacts introduced through PCR
- Multiplex ≤24 samples using Native Barcoding Expansion Packs
The Direct cDNA Sequencing Kit is used to prepare cDNA for nanopore sequencing without using PCR. The kit recommends an input of 100 ng poly-A+RNA.
Taking full-length poly-A+ RNA, complementary strand synthesis and strand switching are performed using kit-supplied oligonucleotides. The RNA template is then digested, and the second (complementary) strand is synthesised. Sequencing adapters supplied in the Direct cDNA Sequencing Kit are then ligated onto the dscDNA.
The Native Barcoding Expansion packs 1-12 (OXNTEXP-NBD104) and 13-24 (OXNTEXP-NBD114) can be used to multiplex up to 24 different RNA samples into a single flow cell.
For sample multiplexing see Native Barcoding expansion packs.
Delivery information: Flow cells and kits are shipped together at 2 to 8 °C. Upon receipt, please place the product in a long-term storage location at ‒20 °C. Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.
Caution: Oxford Nanopore Technologies products are currently for research use only.