Protein Expression competent cell packs efficiently test multiple strains for expression.
- Efficient and economical way to test multiple strains for high protein expression; Contains BL21 and BL21-Gold competent cells
- Directly clone and express most protein constructs
The Protein Expression Competent Cell Pack lets you efficiently economically optimize expression by simultaneously testing multiple strains with your gene of interest.
The pack includes BL21(DE3) competent cells for nontoxic proteins and BL21(DE3) pLysS competent cells for both toxic and nontoxic proteins. DE3 derivatives of BL21 allow protein expression and easy induction. The pack also includes BL21-Gold(DE3) competent cells and BL21 Gold (DE3) pLysS competent cells. The BL21-Gold cells incorporate major improvements and offer higher transformation efficiencies.
BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. Together, these two features allow direct cloning for most protein expression constructs. By cloning directly in the strain, you can save two days of work normally spent on subcloning procedures. The DE3-derivatives of BL21 contain the T7 RNA polymerase gene controlled by the lacUV5 promoter. This all-purpose derivative yields high-level expression and provides easy induction. Use this derivative with nontoxic proteins.
Caution: For research use only. Not for use in diagnostic procedures.