Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/sub-cloning, NGS applications and mutagenesis.
- All-in-one 2x master mix for great convenience
- High fidelity: >60X Taq Fidelity
- 3’→5’ proofreading exonuclease activity
- Processes up to 11 kb from complex templates (eg. Human gDNA)
- Good coverage on DNA templates with low to high GC content
- Fast extension rate: 10 s/kb
Fast HiFi DNA Polymerase 2x master mix is a ready to use 2x reaction mix composed of Fast HiFi DNA Polymerase and an optimised buffer system including dNTPs and Magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets. Fast HiFi DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.
For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 to 2 M Betaine enhancer solution is recommended.
Fast HiFi DNA Polymerase 2x master mix is recommended for applications, which require extremely high fidelity such as cloning/sub-cloning, NGS applications and mutagenesis.
Sample types: gDNA, plasmid DNA, bacterial DNA, λDNA and other DNA templates