Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.
- High fidelity: >60X Taq Fidelity
- 3’→5’ proofreading exonuclease activity
- Processes up to 18 kb from complex templates (eg. Human gDNA)
- Good coverage on DNA templates with low to high GC content
- Fast extension rate: 10 s/kb
Fast HiFi DNA Polymerase 2 U/µl exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches. It is supported with 5X Fast HiFi Buffer allowing robust amplification on DNA targets with low to high GC content and long DNA targets.
For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 to 2 M Betaine enhancer solution is recommended.
For more convenient handling Fast HiFi DNA Polymerase 2 U/µl is also available as a 2X master mix.
Sample types: gDNA, plasmid DNA, bacterial DNA, λDNA and other DNA templates