The kuroGEL 2020Y denaturing gradient gel electrophoresis (DGGE) system is intended for use by researchers studying mutations and DNA polymorphisms critical in disease aetiology and genetic diversity. Based on the kuroGEL 2020 system, the DGGE version is designed primarily for parallel DGGE, where electrophoresis and the denaturing gradient run in the same direction. The kuroGEL 2020Y-DGGE has a maximum 96-sample throughput.
- 96-sample throughput, using two 48-sample combs, allows samples to be transferred quickly and easily from standard microtitre plates or thermal cycler blocks
- Large format, 20,5×20 cm, glass plates enable gradient gels to be poured containing wider ranges of denaturant concentration, maximising sensitivity and resolution
- 100 ml gradient mixer is included for use with the gel running module and casting base to make two 1 mm parallel denaturing gradient gels
- 400 W heater, manually controlled by a temperature control unit connected to a PT100 heat sensor, enables the gel temperature to be set in 1 °C increments to a predetermined melting temperature (Tm) of the PCR amplified DNA polymorphism or mutation of interest (to a maximum of 70 °C)
Gel W×H: 165×175 mm
Unit W×D×H: 285×150×290 mm
Sample capacity: 2×48
Buffer volume, inner chamber: 700 ml
Buffer volume, outer chamber: 4200 ml
Delivery information: Each system includes twin-plate maxi-gel unit with gel running module; heated gel tank with PT100 temperature sensor and thermometer; lid; two 20.5×20 cm plain glass plates, two 20.5×20 cm notched glass plates, four 1 mm spacers, two spacer aligners and two 1.0 mm thick 24-sample combs; casting base; 2 silicone seals; 1 dummy plate; plus GM 100 gradient mixer and heat sensor control unit. Other combs and accessories are available, please contact your local VWR office for details.