FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest green reaction buffers.
- Direct loading on gels and 100% buffer compatibility with downstream applications
- No star activity and 176 FastDigest enzymes available
- Methylation sensitivity: not CpG methylation and dcm methylation-sensitive, dam methylation-sensitive
- Optimal reaction temperature: 37 °C
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5 to 15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as klenow fragment, T4 DNA ligase, alkaline phosphatases and T4 DNA polymerase all have 100% activity in FastDigest buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
Applications include molecular cloning, restriction site mapping, genotyping, restriction fragment length polymorphism (RFLP), and SNP analysis.
Caution: For research use only. Not for use in diagnostic procedures.