peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.
- Comprises of 10 fragments: ~180, ~130, ~95, ~72, ~55, ~43, ~34, ~26, ~15 and ~10 kDa
Eight of the 10 proteins, ranging in apparent molecular weight from approximately 10 to 170 kDa, are coupled with a blue chromophore and resolve into clearly defined, sharp bands when analysed by SDS-PAGE. A 72 kDa protein is coupled with an orange dye and serves as a reference band. The 10 kDa band is green for easier orientation.
The protein marker can be used for approximate size estimation of unknown proteins, however, for precise determination of molecular weights the use of 'unstained' protein markers is recommended. The pre-stained marker is ideal for monitoring the progression of the gel run and Western transfer efficiency.
Caution: peqGOLD Protein Marker IV is optimised for runs on 4 to 20% SDS polyacrylamide gels. 8 to 10% gels may cause proteins with low molecular weights to migrate with the dye front.
Covalently-coupled chromophores affect protein mobility. peqGOLD pre-stained protein marker should be used only for approximate molecular weight determination.
peqGOLD Protein Marker IV contains 2% SDS and is, therefore, not recommended to be used in native polyacrylamide gels for determining native molecular weights of proteins.