Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of DNA probes.
- Replaces dTTP in reactions in which it serves as a substrate for E. coli DNA polymerase
- Produce digoxigenin-labeled DNA probes in a variety of labeling reactions
Digoxigenin-dUTP replaces dTTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Digoxigenin-dUTP can be used to produce digoxigenin-labeled DNA probes in a variety of labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The resulting digoxigenin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots. The probes can also be used for in situ hybridization procedures on fixed cells and tissues. Digoxigenin-labeled probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-labeled probes. The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes or color-producing enzymes.