Kovac's indole reagent for microbiology
Supplier: PanReac AppliChem
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Danger
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A reagent for the detection of indole-positive and indole-negative microorganisms. Some microorganisms can cleave tryptophan which is especially abundant in trypticalle digested peptone to give pyruvic acid, ammonia and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a colour reaction with 4-dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
The strain purity of the organism to be tested must first be established; it is then inoculated into an appropriate culture medium and incubated for 18-24 hours at the optimal incubation temperature. The medium is then covered with a layer of KOVÁCS’ indole reagent of about 0,5 cm. If indole is present the reagent layer turns cherry red in colour after a few minutes.
Ordering information: Contains 50 g 4-(Dimethylamino) benzaldehyde, 250 ml hydrochloric acid 37 % and 750 ml 1-butanol.
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Formula:
(CH₃)₂NC₆H₄CHO MW: 149,19 g/mol Storage Temperature: Refrigerator |
MDL Number:
MFCD00003381 CAS Number: 100-10-7 UN: 2920 ADR: 8 (3),II |
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