Maxima™ H Minus cDNA synthesis master mix

Supplier: Fermentas (Thermo Scientific)
Fermentas
M1661EA 372 EUR
M1661 M1662
Maxima™ H Minus cDNA synthesis master mix
Nucleic Acid Reagents RT-PCR and cDNA Reagents
Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

  • One-tube master mix helps reduce pipetting steps and enhanced consistency in RT-qPCR results
  • Increased RT efficiency across a wide range of input RNA amounts and gene targets
  • High thermostability to allow RT reactions at 50 to 65 °C temperature range

The master mix contains Maxima™ H Minus Reverse Transcriptase (RT) and RiboLock™ RNase Inhibitor. Maxima™ H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution. The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity. The recombinant RiboLock™ RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55 °C. The master mix also contains reaction buffer, dNTPs, oligo (dT)18, and random hexamer primers. A separate tube of No-RT control mix is also provided.

Delivery information: Kit contains Maxima™ H Minus cDNA synthesis master mix, Maxima™ H Minus cDNA synthesis master mix No RT control, and nuclease-free water.
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