CarboxyLink™ Immobilisation Kit is for covalent immobilisation of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.
- Efficient immobilisation
- Stable linkage
- Flexible and gentle coupling conditions
- Ideal for unmodified peptides
The CarboxyLink™ resin included in this kit consists of beaded polyacrylamide UltraLink™ support that has been activated with diamino-dipropylamine (DADPA) to contain long spacer arms, each with a primary amine at the end. When incubated with the resin and the carbodiimide crosslinker EDC (included in the CarboxyLink™ Immobilisation Kit), carboxyl-containing molecules become permanently attached to the support by stable amide bonds. Once a molecule is coupled, the prepared affinity column can be used multiple times in typical protein affinity purification procedures. CarboxyLink™ Coupling Resins can also be used to immobilise other kinds of molecules using alternative amine-reactive crosslinking chemistries.