Immobilisation kit with UltraLink™ support, CarboxyLink™, Pierce™

Supplier: Thermo Scientific
PIER53154EA 624 EUR
PIER53154
Immobilisation kit with UltraLink™ support, CarboxyLink™, Pierce™
CarboxyLink™ Immobilisation Kit is for covalent immobilisation of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

  • Efficient immobilisation
  • Stable linkage
  • Flexible and gentle coupling conditions
  • Ideal for unmodified peptides

The CarboxyLink™ resin included in this kit consists of beaded polyacrylamide UltraLink™ support that has been activated with diamino-dipropylamine (DADPA) to contain long spacer arms, each with a primary amine at the end. When incubated with the resin and the carbodiimide crosslinker EDC (included in the CarboxyLink™ Immobilisation Kit), carboxyl-containing molecules become permanently attached to the support by stable amide bonds. Once a molecule is coupled, the prepared affinity column can be used multiple times in typical protein affinity purification procedures. CarboxyLink™ Coupling Resins can also be used to immobilise other kinds of molecules using alternative amine-reactive crosslinking chemistries.
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