Gaussia Luc vectors for Luciferase assays, Pierce™

Supplier: Thermo Scientific
PIER16147EA 96.75 EUR
PIER16147
Gaussia Luc vectors for Luciferase assays, Pierce™
Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

  • Optimised for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1) and transcriptional pause site (TPS) used to minimise background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin

pMCS-Gaussia Luc vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Gaussia luciferase reporter.

pCMV-Gaussia Luc vector is a derivative of the pMCS-Gaussia Luc vector containing the Gaussia luciferase gene under the control of the CMV (Cytomegalovirus) promoter. Strong (CMV) constitutive promoters for co-transfection and normalisation.

pTK-Gaussia Luc vector is a derivative of the pMCS-Gaussia Luc vector containing the Gaussia luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. Weak (TK) constitutive promoters for co-transfection and normalisation.
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