These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.
- Secondary antibodies are validated for use with the SuperSignal™ West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
- Kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 cm2 membrane)
- Choice of standard kit (with substrate and antibody only) or a complete kit that also includes TBS wash buffer and high performance SuperBlock™ Blocking Buffer