Glutathione spin columns, Pierce™

Supplier: Thermo Scientific
Pierce
PIER16105EA 409 EUR
PIER16105 PIER16104 PIER16103
Glutathione spin columns, Pierce™
Filters Centrifugal Filters
These spin columns provide a fast, easy to use format for purifying GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.

  • Binds at least 40 mg of purified recombinant GST protein per ml of resin
  • Consistently purifies at least 10 mg of GST-tagged protein per ml of resin with greater than 90% purity
  • Resin can be reused at least five times without reduction in binding capacity and purification performance
  • Works well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions
  • Validated and effective for use with Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures
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