Pierce™ O-GlcNAc Western Blot Detection Kit contains a specific antibody for the O-GlcNAc post-translational modification (PTM), as well as detection reagents needed to perform a complete detection experiment.
- Highly specific monoclonal antibody against O-GlcNAc modification
- Speed and sensitivity of chemiluminescent detection
- Specific affinity for the beta-O-linked serine or threonine GlcNAc modification
Delivery information: The kit contains sufficient reagents for approximately 10 mini blots:
Part A:
M-PER™ Mammalian Protein Extraction Reagent, 25 ml
Dilution Buffer (also functions as Blocking Buffer) (10X), 2×50 ml
BupH™ Phosphate Buffered Saline, 17 packs
Surfact-Amps™ 20 (10% Tween™ 20 Detergent), 3×10 ml
Goat Anti-Mouse IgM (µ), HRP Conjugate, 75 µg
SuperSignal™ West Dura Luminol/Enhancer Solution, 50 ml
SuperSignal™ West Dura Stable Peroxide Solution, 50 ml
Part B:
Anti-O-GlcNAc Monoclonal Antibody (MAb CTD 110.6), 100 µl
O-GlcNAc Western Blot Positive Control (O-GlcNAc-modified BSA), 100 ng