XChange Plasmid Midi Kit, peqGOLD

PEQLEMR508020EA 101 EUR
PEQLEMR508020 PEQLEMR508010 PEQL12-7401-02 PEQL12-7401-01
XChange Plasmid Midi Kit, peqGOLD
XChange Plasmid Midi Kit for the isolation of up to 100 µg plasmid DNA per preparation. Anion exchange technology ensures highly pure and intact plasmids up to 300 kbp.

  • Quick and easy protocol - short hands-on and preparation times of only 65 minutes
  • No need for centrifugation steps effectively protects DNA during extraction
  • Complete enzymatic degradation of all cellular RNA and nucleases
  • Efficient removal of enzyme inhibitors and contaminants
  • Plasmid DNA is suitable for high transfection efficiencies

The peqGOLD XChange Plasmid Midi kits combine a modified method of alkaline lysis with the DNA binding properties of XChange Anion exchange gels. An optimised packing process leads to a porous column matrix where bacterial lysates or Wash and Elution buffers can rapidly and consistently pass through by gravitation. The column matrix contains positively charged active groups which bind the negatively charged DNA while enzyme inhibitors and contaminants are easily washed through. After the washing step the pure plasmid DNA is eluted and then alcohol precipitated. Although flow rates are rapid and protocol times are short, the isolated DNA is highly pure, provides excellent chromatographic definition and thus is ready to use for a wide range of downstream applications.

Delivery information: Each kit includes XChange Midi columns, folded filters, plastic washers, Solution I, II and III, EQ Buffer, DNA Wash Buffer, RNase A, and Elution Buffer.
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