This kit isolates proteins from both the cytoplasmic and nuclear fractions of cultured cells. The cytoplasm and the nucleus are separated from one another by simple centrifugation steps following lysis in a non-ionic detergent. The entire procedure can be performed in less than 1,5 hours.
- Generate distinct nuclear and cytoplasmic fractions from cultured cells in 90 minutes
- For adherent or non-adherent cultured cells
- Compatible with downstream Western blotting applications
Delivery information: Kit includes Fractionation Buffer 1 (1 ml), Fractionation Buffer 2 (1,5 ml), Fractionation Buffer 3 (30 ml), and Nonidet® P-40 Substitute (0,1 ml). Contains sufficient material for 20 extractions of 1×10⁶ cells.
